Importantly, selective up-regulated clones showed characteristic cultivar-and tissue-specific expression profiles. Global transcript profiling by reverse northern analysis revealed that a large number of clones from these libraries were up-regulated by anaerobic stress. Results In all, 400 and 606 cDNA clones were obtained from the SSL and DSL, respectively.The DSL was constructed from mRNAs isolated from the roots of low O₂-stressed FR13A rice seedlings from which pools of low-O₂-stress up-regulated mRNAs from Pusa Basmati 1 and constitutively expressed mRNAs from FR13A roots were subtracted. Methods To construct the SSL, mRNAs present in the unstressed control FR13A roots were subtracted from the mRNA pool present in low O₂-stressed roots of FR13A rice seedlings.With the aim of isolating genes that might be specifically involved in flooding stress-tolerance mechanism(s), two subtractive cDNA libraries for the flooding-stress-tolerant rice genotype FR13A have been constructed, namely the single and double subtraction libraries (SSL and DSL, respectively). However, specific genes are noted to be up-regulated in this response. Background and Aims Flooding stress leads to a significant reduction in transcription and translation of genes involved in basal metabolism of plants.
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